EbEST: An Automated Tool Using Expressed Sequence Tags to Delineate Gene Structure
نویسندگان
چکیده
منابع مشابه
Expressed Sequence Tags
Now a days, genome analysis has employed a rapid analysis tool known as expressed sequence tags. In 1983, SD Putney for the first time demonstrated the use of cDNA in identification of genome. The term expressed sequence tags (ESTs) was coined by Anthony Kerlavage at the Institute for Genomic Research. In 1991, Mark Adams used EST in relation to gene discovery and Human Genome project. cDNA or ...
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Understanding which genes are active, and when and why, is an important question for molecular biology. Expressed Sequence Tags (ESTs) are a technology used to explore the transcriptome (a record of this gene activity). ESTs are short fragments of DNA created in the laboratory from mRNA extracted from a cell. The key computational step in their processing is clustering : putting all ESTs associ...
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To further increase our understanding of responses in potato to abiotic stress and the potato transcriptome in general, we generated 20 756 expressed sequence tags (ESTs) from a cDNA library constructed by pooling mRNA from heat-, cold-, salt-, and drought-stressed potato leaves and roots. These ESTs were clustered and assembled into a collection of 5240 unique sequences with 3344 contigs and 1...
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MOTIVATION Expressed Sequence Tags (ESTs) are short single-pass DNA sequences obtained from either ends of cDNA clones. To exploit these sequences efficiently, a dynamic Web-tool has been developed which uses these data to perform fast virtual cloning of cDNAs. RESULTS Starting with a query sequence, the user is able to identify related ESTs and extend the sequence of interest step by step, p...
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Coffee is one of the most important commodities in the world, and its production relies mainly on two species, Coffea arabica and Coffea canephora. Although there are diverse transcriptome datasets available for coffee trees, few research groups have exploited the potential knowledge contained in these data, especially with respect to fruit and seed development. Here, we present a comparative a...
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ژورنال
عنوان ژورنال: Genome Research
سال: 1998
ISSN: 1088-9051,1549-5469
DOI: 10.1101/gr.8.3.268